Entire lungs were perfused with 20 ml PBS via center puncture before preparing single-cell suspensions with an automatic tissues dissociator (gentleMACS; Miltenyi Biotec). For enumeration of lung immune system infiltrates by stream cytometry, cells were ready as described previously (Van Dyken et al., 2014), using antibodies shown in Key Assets and gating as defined in … Continue reading Entire lungs were perfused with 20 ml PBS via center puncture before preparing single-cell suspensions with an automatic tissues dissociator (gentleMACS; Miltenyi Biotec)